LOCUS EY189856 546 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa02a05.b1 R.similis_EST_RSAA Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY189856 VERSION EY189856.1 GI:159491887 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 546) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 52 original value before phred recall for SR was 622 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..546 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgttcgct tggggggttt tttttttttt ttttggttgt cagagttctt 61 tgggggggat atgcgattgc caaattggag ttgtggatcc tggctgaatc gtgggtggtg 121 ttcagtgtcc actgttcgct tccccttcgc tttgcgctca tttcgatttg gagcccttgg 181 ccccgaacgg gtagtacgag tagtacgggt tgtactggta ctgctggtag tagtacgggt 241 acgaagacgg gtccgcactc acgtacccgt atccaggcac gtacacggag ccgctgttcg 301 tcctgtaggt gccgctgact gggtcgtacg tgtagtaggt cccgctgctc gggtcgtagt 361 agttgtactg gctggacgag taggagggcg agtaggacgg atagtagtag gacgggtacg 421 cggacgggta ctgctggtag tagtaggttg ccccttgtgc ggccgagtgc agttgccaga 481 gggccagagc gacgagcaga agggtagtgg ctgtcgccaa cctggtgcgc atggccattt 541 ggaaag //