LOCUS       EY189841                 441 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01g09.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to emb|CAB59358.1| translation elongation factor eEF-1
            alpha chain [Anisakis simplex], mRNA sequence.
ACCESSION   EY189841
VERSION     EY189841.1  GI:159491872
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 46
            original value before phred recall for SR was 484
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttgtggtttt tttttttttt ttttggcaaa ataaacttct
       61 ttaaggtaac gacaatatga tagaggacaa cgatgaacaa agaaagagtg tcaagagatg
      121 gtggtgcaat ctttgcgtcc ctcaaagacc gtgtcctcat tcgttacttc ttcttggccg
      181 gagcggcgcc agccttctgt gcagacttgg tgacctttcc gccgccctcc gacttctcga
      241 cgcccttgat cactccgaca gccaccgtct gtctcatgtc gcggacggcg aagcgtccca
      301 ggggagcgta gtcggtgaac gcctccacgc acatcggctt ggtcgggacc agctccacga
      361 tgccggcgtc tccggacttg agtgccttcg ggttgtcctc caccttcttg ccggaacgac
      421 ggtccacctt ccccggccgt a
//