LOCUS EY189840 444 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa01g08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY189840 VERSION EY189840.1 GI:159491871 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 444) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 58 original value before phred recall for SR was 498 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..444 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgttcctt taaggggtct tttttttttt tgtgtggtgc aagccacctt 61 taaggagaaa caatggatct tgtgatgttt gatctgagcc taagaaattg aagggagacg 121 actgcctgtt taatgttgat gcacttgaat atgagtttga gcaatgagat cacttcttcc 181 tttgttgcct ttcggcagct tcaaacagtt ctgtgagcga tgcatagttc tctcccccgt 241 cctttgctat ccggaagcgg tcgccgaaca tgtttgtcat tttgcggccg aactgcttcc 301 agctgtcaac gccgagcatt tccgagacga ggatcacgaa cgtcaaatag ccggacactg 361 tgatcactgt tgcgatgccg agcgcttgca tggcgatgct ggaaccggtg agaatgcccc 421 gtctggtcag ttctcccggc cgta //