LOCUS       EY189838                 377 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01g06.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to emb|CAA70477.2| SEC-2 protein [Globodera pallida], mRNA
            sequence.
ACCESSION   EY189838
VERSION     EY189838.1  GI:159491869
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 60
            original value before phred recall for SR was 435
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttctat gaggggtttt tttttttttt ttttggctct cgggtccctt
       61 ttaggggtgg ccggatcaga acaggctagc gcaaaaagtt ggttatttat gtggggcaac
      121 tgatactgat tttatttgtt tctcttcgtt ctctcaatgt tcacttcctt ccttcagctt
      181 ctgcttaggc ggcggtggcc tccggcttca gcagactctg ggccaacttc tggaacttct
      241 cgttttggat gacgccggtg atcttgggga aattgctctt caaggactcc ttcgcctcct
      301 cgctcagtgc cttgtacttc tcgatgatct cgttggcctg cttgcgcagt tcctccaggt
      361 tcggcttccc ggccgta
//