LOCUS       EY189835                 519 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01g01.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to emb|CAA74624.1| elongation factor-1d [Sphaerechinus
            granularis] emb|CAA74625.1| elongation factor-1d [Sphaerechinus
            granularis], mRNA sequence.
ACCESSION   EY189835
VERSION     EY189835.1  GI:159491866
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 569
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtcaatc gagggccctt tttttttttt tttccacttt tgaactcttt
       61 aagggggtgc cataaaccaa caaaaaccaa atgtcaacaa tgcgagtggc caacaaatgt
      121 gtgatatttt ttcccaaacc aagcctgcga aagtcaataa taggctcctg ctgctcatca
      181 atcgctcatc agactttgtt gaaggcgacc acatcgaccg attggacgag gtcctcaaag
      241 ccagtgatct gctcctctag ccagtcagtt gagacctttt cgtcctccac acagcagcaa
      301 atctgaagct tcttaacgcc gaatgccact ggcaggatct tggaagtgcc ccagacaagc
      361 ccctctgtgt gaatagagcg cacattcttc tcgatctcct tgatgtccac agtgtcgtcc
      421 cagggcttga catcgtaaat cacgttggac ttggcgatgg ggccgggctt cttggacttc
      481 ttctccgcgt aggccttcaa gcgctcctcc ccggccgta
//