LOCUS       EY189806                 375 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01d04.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to gb|AAK31158.1|AF348330_4 N utilization substance protein
            A [Wolbachia sp. wRi], mRNA sequence.
ACCESSION   EY189806
VERSION     EY189806.1  GI:159491837
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 872 was extended to value 872 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 497
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 tttccttttt gccaacacac gccccaaccg gatcaatatt tttatcagga gagaaaacag
       61 caacttttga tctcgatcca gcatctctca ctgtagcttt aattaccaca agtccatcag
      121 taatttctgg tacttcttgc tttaacaatg cttctaagaa tcctggatgt gtacgagata
      181 gaatgacttg tcgtccatca tcagaacgtc tgacactttg tatataagcc ttaatcttat
      241 caccttcacg aaatctctcg tcacctatta aattctttaa cgaaagaaat gcagtggtac
      301 catttatatc aacaataagg tctggatatc ctacttgctt aacaatacca taccttactt
      361 ctccacctgg ccgta
//