LOCUS       EY189782                 583 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa01a08.b1 R.similis_EST_RSAA Radopholus similis cDNA 3'
            similar to ref|NP_495449.1| Cys Met metabolism PLP-dependent enzyme
            (43.1 kD) [Caenorhabditis elegans] sp|P55216|CGL_CAEEL PUTATIVE
            CYSTATHIONINE GAMMA-LYASE (GAMMA-CYSTATHIONASE) pir|C88197 protein
            ZK1127.10 [imported] - Caenorhabditis elegans gb|AAB93432, mRNA
            sequence.
ACCESSION   EY189782
VERSION     EY189782.1  GI:159491813
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 598 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 53
            original value before phred recall for SR was 651
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcctt aagggggttt tttttttttt tttcattcca ctggtttctt
       61 taaggggcat acacaagtgc aacgcgttct tgccttttag tgtatcctaa gcactgccag
      121 catttccaat ttccgcctcc agtgcctggt ccaagtcgtg gagcaggtcc tccacgtcct
      181 cgcagcccac cgacagtcga atcaggttgc tcccgatgcc gagctggccg cgcacttctg
      241 ggggcacatg tgcctgaaac tgggcagcgg gcatgctgac caacgactcg tatccgccca
      301 gagaagtggc cgacttgaac acctttaggc tggccaagaa cttttcggat gcttcgcgtt
      361 tgctggagtg caggtagcac gagaccatgc cggacatgcc gctgcattgg cgcttgtgca
      421 cctcgtgctg gggatgcgac tccagcgccg gatagagcac acgggccacg cgcacatctt
      481 tctccaggaa gcgcgccacg gcgagcccat tcgccgagtg ctggcgcatc cggagatgca
      541 ttgttttgat gccgcccaag accaaatatg tgtcaaaggg gga
//