LOCUS       CV130281                 164 bp    mRNA    linear   EST 02-SEP-2004
DEFINITION  pe24e07.y2 Radopholus similis pAMP1 v1 Radopholus similis cDNA 5'
            similar to contains element TAR1 repetitive element, mRNA sequence.
ACCESSION   CV130281
VERSION     CV130281.1  GI:51865801
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 164)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: -40RP from Gibco
            High quality sequence stop: 156.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016319 Radopholus similis pAMP1 v1"
                     /dev_stage="Mixed"
                     /lab_host="DH10B"
                     /note="Vector: pAMP1 (gibco); Site_1: 5': SalI; Site_2:
                     3': NotI; The library was constructed by Irina Ronko and
                     Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at
                     Washington University, St. Louis. The cDNA was made by
                     using Dynabead oligo-dT priming (Dynal). PCR based library
                     using a modified protocolfrom the SMART PCR cDNA Synthesis
                     Kit from Clontech. Directionally cloned into the UDG sites
                     of pAMP1. Radopholus similis total RNA was provided by Dr.
                     Godelieve Gheysen of Ghent University
                     (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcaccgccgc caaggcagcg agaccgctta ccggtcggca ggaaaaggtc tagaacacca
       61 ccgagaaggc ggtcgccgcc agactctcat aggagtcagc caggggatca ccgtcgagaa
      121 agacgccggt ctccattgga acgggaggaa aggttgnggc ggga
//