LOCUS       CO961498                 620 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18036 Q18036 SIMILAR TO NADH-UBIQUINONE
            OXIDOREDUCTASE B22. [1], mRNA sequence.
ACCESSION   CO961498
VERSION     CO961498.1  GI:51334682
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 616.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttactttgag tttgattctt tcgtatttta catttcaccg caatggtgct tccgtctatg
       61 gattcgcccg cttggatgtt taccaaggcc ctctctcatc gtcaaaaagt gatgcgactt
      121 tacaaaagat gcattcgaga ggttgatgcc tggcacggaa ctgaattttt ggaaaaacat
      181 ttccaaagag tactcatgcg ggcccgtttc gatgccaata aggacgttac ggatatgagg
      241 ctgtcgcaat atttgctcgc agatggatgt cgacagctct ggcagaatcg acatgtaaag
      301 ccggtgagat ttgcaaatga tccccttgga agcaattatg atcgtgaaat ggaggccgac
      361 gacatgcttt tggaagacaa cgtacaatgg acttgggcag aaaaagaaca gttcccttac
      421 tacttcaatc gacgagaaaa gcgcaagaag gagttaatgg cgcattggca taaaatcgag
      481 gccggctggg atgcggaatt ggacgccatc cagcgagaac tgccaaaagg gggagagaac
      541 agcggaaagc agaatggagg tgttaaaggt gtcctgaagt gagacgacgg ttgaaggaag
      601 ggatggatgt ggattgngga
//