LOCUS       CO961491                 471 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22g10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:Y248_CAEEL O76387 HYPOTHETICAL 33.7 KDA PROTEIN
            C24G6.8 IN CHROMOSOME V. [1], mRNA sequence.
ACCESSION   CO961491
VERSION     CO961491.1  GI:51334675
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 472
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcgttctttc tgcggccgag actcaactga ccgctggacc gcacaaaatg gtctttgtcg
       61 catatatggg attaaaaatg ggcgcaggta aactggcagc acaggtgggc catgctgcgg
      121 tggcacttta caggacagca caaaagtcac gagaaggaca agtggccctg gattattgga
      181 agaggcatgg cgagatgaag attgtgctca aaggaaaaaa tgcggaagag ctcgcggaat
      241 tgttcggtga agcgagaaga gctggtcttt ttgcctacat tgttgcggac gcaggccaca
      301 cacaaatagc ggcaggttcc cgcacaattc tgggcatatt cggtacacac gaagaagttg
      361 acaagataac cggacactta aagctgctct aacatttcct gtttctttgc gacttatctg
      421 attgtgcatt tgtgaacact gtgaaatcat ggaaaaaaaa aaaaaaacaa a
//