LOCUS       CO961490                 543 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22g09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YOT9_CAEEL P34654 HYPOTHETICAL 13.4 KD PROTEIN
            ZK632.9 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO961490
VERSION     CO961490.1  GI:51334674
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aacagtcttc atacccaaac cacgatggca tcaaaccaat ccgccgccga actggccgag
       61 gagtacgagc gtggagagca cgacgaagtc cccaagagtg gccagggcaa gcgctccaag
      121 gcagaacagg tggagcacaa gcacgcgcac ccggaggggg acacgcgtcg catcacggac
      181 aatgcggtga agggcgagga gaaacgcaag gaggaggagc gcaagcgcac caactcccag
      241 tccaagtgag cggcggagga gaacgacgga agcgtgtgtg tgtgccttgt tgaataactg
      301 aacttccttc actttcattt tcgagatatt ccccgcacac gctgacggcc aacaaattcc
      361 atctttccac aaaaacaatc ctcgatccac acacagattt tgttaagcag aagaataaag
      421 agccattgtg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaccaaa accccaaaac aattttcccc ccaggggaaa
      541 att
//