LOCUS       CO961471                 595 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YKAE_CAEEL P34264 PROBABLE RIBOSOMAL PROTEIN
            B0303.15 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO961471
VERSION     CO961471.1  GI:51334655
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 664
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gaggcaataa aggttgtgct tgatccgatc ctaaagacgc agattagggc acagatggcc
       61 tctgccgctc cgcctctggg tccttcgctg gggcagcgtg gagtgaacgt ggcaaatttc
      121 tgcaaagact tcaacagttc gactgcgcac atcaaacaag gcactatttt gcccgtgaca
      181 gtgcatgtga agccggacag aagttacaat ctcgagatcg actcgccggc tacaacgtgg
      241 ctgctgaaac aggcggcggg aattagacgg gacaaacagg aggcaggcga aatcgtcgga
      301 cggctttctg tcaaacatat ttacgaaatt gcaaagatca agtccaaaga caagtctctc
      361 gttggtgttc ccctgaggga tatctgccag atggtcatcc aaagggctca gacaattggc
      421 atcgccgtcc aaaacgagga cttggatcca cagcggtata aggaattttt ggacacacga
      481 agggaaattg tgaaacgaca attggaggag ataaccaaaa aggaacaagc aaaattgctt
      541 agggcttaac tccttttccg caatcaattg ttgtggataa acctagaaat cttgc
//