LOCUS       CO961462                 683 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22d07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q20637 Q20637 F52A8.1 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961462
VERSION     CO961462.1  GI:51334646
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 711
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agttctgtat gattggcttg ctgttgtgcg ttgttgcttg tggagatgtg tgcggggaga
       61 aaacactgtt ggatgagttg cgcgacttgg aaatctttca acttcgtagc ggcatatcgg
      121 catgccccct gcctttggtg ggggaaccgt gccctcagtc caatgccatc ttctacttcc
      181 gctgttgcgg acagctaaac gagagctgct gcttccgcct tcaagactgg ctcgtcgctt
      241 tgatcgcact gtttttggtg cttatcatcc tcagttgctt cgtcaatctg atccgctgtc
      301 tcttctgcag caactactga tgggatcgag ttacgcacaa tggaaaaggc catttcttgc
      361 tcaccaattc gccgttatca aacttctttc aaccatttta cttattcaat taacttttct
      421 ccaattatag ctaatgggaa caaagaatct taaacaaatc ccaatctatt cttcaagcaa
      481 tcacatattt ttaaatattt tctgtgctct tcaatcatac cgataactct cgatcgcatc
      541 atatttgtaa tgctataata tataactcac gaccaatcaa ctacgaatgc gtatgttgtt
      601 tgttactttc actgacacat ttcttgactt gaaataaaac attcttcctg aaaaaaaaaa
      661 aaaaaaaaaa aaaaaaaaaa aaa
//