LOCUS       CO961460                 450 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22d04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90849 P90849 F26E4.9 PROTEIN. [1] ;contains
            MER22.t1 MSR1 repetitive element, mRNA sequence.
ACCESSION   CO961460
VERSION     CO961460.1  GI:51334644
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcgaaagca cgctttcaat tgaccacact tggtacgaac aagcgttttt tcgcttcgga
       61 ggctgatccc gatgactacg gttaccaccc cgatccgatt gaacacgctt ggggacggga
      121 aaggaaaatg atcattgcac gtctgatggg cgacgatcgt tacgaaccca aagtgtatta
      181 tcgcgcagag gaatccacac gagacatgcc aaatcttgtg ccctgtcact ttgacgacaa
      241 ggtggtcggc tgcttgtgcg agccagacac tggatacgtg aactactggg ttatccggaa
      301 gggcaacata aaacggtgtg aatgcgggca ttggttcaaa gggatcgaag ccgatccaga
      361 aagtgtctaa ggacatgcac aacttgatag gacacgtcgg tttattgagt ttgtaaatct
      421 taaagttttc attgataaag tgtttttagc
//