LOCUS       CO961459                 419 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22d03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL2A_RAT P18445 60S RIBOSOMAL PROTEIN L27A, mRNA
            sequence.
ACCESSION   CO961459
VERSION     CO961459.1  GI:51334643
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 1001
            Seq primer: SL1 primer
            High quality sequence stop: 404.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cacgaaagct ccgcggacac gtctcgcatg gacacgggcg catcgggaag caccgcaagc
       61 acccgggcgg tcgcggaaac gccggtggcc agcaccacca ccggatcaac ttcgacaagt
      121 accacccagg gtacttcggg aaggtcggga tgcgcaactt ccacgtgcgc aagaaccact
      181 actactgtcc gacgatcaac ctggacaagg tgtggtccct ggtcacggag cggacgcgcg
      241 ccatgtacgc ggacgtgaag gacaatgcgc cggtgattga cgtagtgcgg gcgggctacc
      301 acaaggtgct gggcaaggga ctgctgccca agcagccggt gatcgtcaag gccgagtact
      361 tctcgcactc ggccgaagag aagatccaga aagcgggcgg cgcatgtgtg ctactctcc
//