LOCUS       CO961453                 441 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22c07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01504 O01504 SIMILARITY TO 60S ACIDIC RIBOSOMAL
            PROTEIN P2. [1], mRNA sequence.
ACCESSION   CO961453
VERSION     CO961453.1  GI:51334637
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 461
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atcaaaatga agtacgtagc tgcctacttg ttggctggtc tcggcggaaa cgcgagccca
       61 tcggccaagg acttggagaa gatcctgggc gccggcaaac tcgacgtgga catggagaac
      121 gcgaacgcgg tgatcaacgc gctcaagggc aagaccgtcg aggaggtgat cgcggccggc
      181 gagaagaagc tggccagcgt gcccagcgga gctccggccg ccgcagcgcc agcggcggca
      241 gcaccggccg cagctgtagc tgagaagaag gaggagaaaa aggaagagaa gaaagaggag
      301 agtgaggacg aggacatggg cttcggactg ttcgattgaa cgcgcgacca ttcgcaaaaa
      361 gagcgtccac tcaaaccgtt gttttcgttt aaaccactat aaatttgttg aacaaaaaag
      421 aagagggaga aaaaaacccc c
//