LOCUS       CO961450                 582 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22c03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS14_CAEEL P48150 PROBABLE 40S RIBOSOMAL PROTEIN
            S14. [1], mRNA sequence.
ACCESSION   CO961450
VERSION     CO961450.1  GI:51334634
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 593
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagctacaat gggctgcccg caaaggaaag gtcaaggaag agcagcccgt tgttacattg
       61 ggtccacaag tgcgagaagg cgagaatgtt ttcggcgtgg cgcacatctt cgcgtccttc
      121 aacgacacct tcgtccacgt gacggatttg tccggtcgcg agacaatcgt caagatcacc
      181 ggtggaatgc gagtgaaggc cgaccgagac gaagccagtc cttatgcggc catgcttgct
      241 gcgcaagatg tggcggagcg ctgtaaacag gtgggggtta atgccctgca cgtcaaactg
      301 cgtgccactg ggggtacaaa gaccaagacg ccaggaccag gcgcccaggc agcgcttcgt
      361 gctcttgctc gttccggcat gaagatcggt cggatcgagg atgtgacgcc gattccgact
      421 gacaccactc gccgaaaggg cggacgtcgt ggacgccgtc tctgagcgct gagaattgtg
      481 gaggagttac agcaatgtta ttgttatttt cgaattgatt ttcgaataaa acgtgcttgt
      541 tttccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaacc aa
//