LOCUS       CO961449                 510 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22c02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VTE0 Q9VTE0 CG14145 PROTEIN, mRNA sequence.
ACCESSION   CO961449
VERSION     CO961449.1  GI:51334633
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 511
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagagcatcg acgagtgtgg aatgtcctct ttcgccaacg gaaccgggga atgcggtagg
       61 agacgaagta agaagacgcg cagcagaggc tgcttccaaa ctctactcgt atatccatgg
      121 acaaatgcgg acaacagtgg acgaatacaa attgttacag gatatcaacg ttaagacggt
      181 gcaacgatac attgacatgg aagaattgac caatacaatt tcaagccggt tgactgggct
      241 acagaccaaa tacgaacaga tgcgccccat actcgaacaa atcaatgaaa ttgaagaagc
      301 tagtagaaga ttggaaactg cagtagccac cttagaggga tatctggatg ctttagaagc
      361 gaagctacga acactacaac gacagaatat gaccatataa atccaaaaaa tatttttatt
      421 gattatcaaa agaatcatgt tcccgacaaa atttccatga accgactcat ttcataataa
      481 taactcatca atgaaaaaaa aaaaaacaaa
//