LOCUS       CO961448                 435 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22c01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O18236 O18236 Y57G11C.12 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961448
VERSION     CO961448.1  GI:51334632
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 503
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 acacgagagg aggcccgcca tcgtgtactc aatttgtaca aaaaggtgca gagagatgct
       61 cccaatatgt ggtggctgta caggatgcaa gatatcccac ttccggtgtt tcnagaagcg
      121 ctcaaaaagc aattctcgaa gaattcgcaa ttgtcagact tgagagtaat cgaccgtaaa
      181 ctagaagaag gtcgtcaaca catggtctcg gtgaaatact tctattataa tgaatatcat
      241 gtgaggaaca tgctgtttgc cgaaaacgta gagccgaaac cgaaagactt cctttccaag
      301 tttctgttgg gcaaagagtg acgagagcag acagcagaga gattctttag gatacctaat
      361 gcaaaaattg acttttttga tgcaaagtca gctctgtata caacacacat ttgataaata
      421 aaaaaaaaaa acaaa
//