LOCUS       CO961447                 637 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22b12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9V832 Q9V832 CG4866 PROTEIN, mRNA sequence.
ACCESSION   CO961447
VERSION     CO961447.1  GI:51334631
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 638
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtaagctga aaatatcatg agaagaaact gctgaagaaa gtcgacttca tccactggcc
       61 ggaggtcgac aacagtttgc acgagaacaa gataatgcgg cgctaccacg tggaaaagag
      121 ggagcactac accttgtaca acaaattgtc gcgacaggtc cgcgagttga cgaagaggat
      181 ccgggatttg gacgacagca accggaacaa gttggccctc gcgaggcagc tcctcggcaa
      241 actctacgac ctgggcatta tccagacggc ggacacgctg gagcggtgcg acagagtcag
      301 tgcctcttcg ttctgcagaa gacgactccc cgtcgttctc ttcaaaatgc aaatggcgcc
      361 aagtgtgaag aaggcgagtg aactggtgga acaggggcac gtgagggtcg gcgtcgagat
      421 ggtcaccgac cccgcgtttc tggtcaccag gacgcagtcc gaccacatca cctggacgag
      481 ggggtcgaag gtgaagaagc atgtgctcgc ctacaacaac gaactggatg atttcgacga
      541 ttagccgaaa ggccgtagaa ttgttgtcgg tcattttacg ctctcaataa atgttctagt
      601 aaattaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//