LOCUS       CO961443                 542 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22b08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO961443
VERSION     CO961443.1  GI:51334627
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 667
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gatcaagaaa atgagcggcg agaatatcag caccaacggc gaccgtgaga acttctacaa
       61 attgctaaaa gacggcacac tactgtgcaa gctggccaac gggattcagg cgggctccgt
      121 caagaagatc cagcagccga ctggcaactt cgcctgcatg gagaacatca acgctttcgt
      181 gtcgttcgcg aagagccagg gagtgcccac cgaggagacc ttccagagcg tggacttgtt
      241 cgaggcacgt gacctctact ccgtgtgcat gaccctgttg tcgttgggcc gaattatgga
      301 gaagaaggga aagccgaacc cattctctgg atgaagagta gaagtgagtg cagcaaaaga
      361 cggacagagc atgtgctatc gctcccattc gagaactccc cgttttgcga attttctccc
      421 cgtgtgcgac cttgcaacaa taccgaggcg ttaactgttt tcccctctct ctcctctcaa
      481 actgtgggca tttgaaaata gcgatgccgg aataaatggc caattcaaaa aaaaaaaaaa
      541 aa
//