LOCUS       CO961437                 499 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22b01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961437
VERSION     CO961437.1  GI:51334621
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 622
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caaacaattt gagcagcgtg gtataaactg gtggctatca aatggtcaaa gcaacgcccc
       61 aacacttgga agtgcttatg aggatctgaa ggaaaacctt tcttcgctgg attagtgaag
      121 tacatggctt cagggccagt tgtggcaatg gtttgggaag gactcgatgt tgtgaaacaa
      181 ggtcgagcta tgctcggagc cacaaatcca ctggtgagtg cagcaggaac aattcgtgga
      241 agctactgta tggacatggg acgaaacatc tgccacggat cagacagtgt tccatcggca
      301 gagcgtgaaa ttgcacactg gttcaagcag gaagaattgg ttgactggga gccagcacag
      361 atcaactgga tctacgagtg aggaacaatc tggaagacgc ccatctgcga taaggaagaa
      421 agtcccggat accaattcac aagaccattt gatttcgagg atttcttatt cttattgtgt
      481 cgaaacttaa agttgacaa
//