LOCUS       CO961434                 473 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22a10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O70378 O70378 HYPOTHETICAL 23.3 KD PROTEIN, mRNA
            sequence.
ACCESSION   CO961434
VERSION     CO961434.1  GI:51334618
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccgcgtactg caagatggtc ctgcacgcca tcaagtaccc gcactgtgca atcaggggcg
       61 ttctgttggg acatcggccc ccgcccaagg gcggggattc ggacgccgag cgaccagaac
      121 ggccattgcg cgtggttgac gccattccgg cgacgcacac ggccctgttg gcgccccccg
      181 tagagacgct gttcatccac ttggacgcct attgccggga gcaacagctc cagattgtgg
      241 gactgtactt ctgcaaccag ctgctggcca acaacagttt ggacgagctc tgggcccgaa
      301 taggcgctga caagatacgc gatccggtgc tgctgcaaat cgacaatgcg aagctgtcgt
      361 tgaacgccac ttcgccgtgt atccgcgctt tcgagccgga aagcgcgggc aagtggcgcc
      421 gggtggagag ctgggcggtg gacaattccc gaccactccg tgatcctggc gtc
//