LOCUS       CO961430                 495 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22a06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL17_RAT P24049 60S RIBOSOMAL PROTEIN L17, mRNA
            sequence.
ACCESSION   CO961430
VERSION     CO961430.1  GI:51334614
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aaggcccgcg gctcagattc tccgggtgca cttcaagaac acccatgaga ctgctcgggc
       61 catcaagcac atggcgctga agcgggcgat ccgcttcttg gagaatgtca aggaaaagaa
      121 ggagatgtac cattccgtcg cttcaagggc ggtgttggac gaaaggcgca ggcaaaagcc
      181 tggggcacta gccaaggtcg ctggcccaag aagtccgccg agttcctgct ccagctcctc
      241 agaaatgctg agagcaacgc cgaggtcaag ggcctggatg tggaccacct ggtgatcgac
      301 cacattgtgg tccaggccgc ggccaaaatg cgcagacgca cctaccgtgc ccatggccgc
      361 atcaacccgt acatgagctc gccatgccac atcgaagtga tcctgaccga gaaaaaggag
      421 gtcgtctcca aaccggccgg tgcggtggtc aaggtgaaga aggagtccaa gacgaagcac
      481 cgacgccagc tcgcc
//