LOCUS       CO961428                 595 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22a04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9SLI9 Q9SLI9 F20D21.26 PROTEIN, mRNA sequence.
ACCESSION   CO961428
VERSION     CO961428.1  GI:51334612
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 696
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gaattctcac caaatgcaca aattaacgaa aaaactgcac aagacacgga tgttcgcgtt
       61 caacgtggaa cacacgtgca tgatccacac aaaggaacag tttgtttgtc tgctgcaagt
      121 gtccactcgc caccacgatt acattattga tctgctcaat gagcgtggtc gtccatcgcg
      181 gcggatggca cagcacatga gtctgctgag agaagcattc gcagataagc gcattcggaa
      241 ggtcgtccat ggctcgcagg acaccaaatg gctgatggac tatttcggca ttaaaactgc
      301 caactgccac aacaccaatg caggtggaat tggattgaag gtgctggtcg agcgcgagtg
      361 cggtgtgcag ttggacaagc ggggtcagaa gtggaactgg gcgcgacggc ccctcacttc
      421 ggccatgatg cactatgcca gatgcgacac ccatttcttg tacagggcct accgacagat
      481 ggaaaagcga tcgttttgaa agtattccat ttagaattta gacaaaagag aaatggcaaa
      541 taatttaaaa taaagttcgt gaagcaaaaa aaaaaaaaaa aaaaaaaaaa acaaa
//