LOCUS       CO961426                 746 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe22a02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS5_CAEEL P49041 PROBABLE 40S RIBOSOMAL PROTEIN
            S5. [1], mRNA sequence.
ACCESSION   CO961426
VERSION     CO961426.1  GI:51334610
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcctctgaa gttgaaggaa tcccccggcg gacacttgga atgcggaaga ggttgtggat
       61 gagggtgcca tggagcactt ggtgagcatt gatgttccgg aggtgaagct gttcggcaaa
      121 tggtcactca acgatgtgga ggtctcagac atctcgctcg tggactacat tgccgtgaaa
      181 gaaaaagcgg ccaagtactt gccccacagc gccggtcgct accaggtgaa gcgcttccgc
      241 aaagccactt gccccgttgt ggagcgtctg gtctcctcga tgatgatgca cggccgcaat
      301 aatggaaaaa agctgatgac agtgcggatt gtcaaacacg ctttcgagat catccacctg
      361 ctaactggcg agaatcctgt gcaagtgctc ataaacgccg tgatcaacag tgggccacgc
      421 gaagactcga cacgtatcgg tcgggctgga actgttcggc ggcaggccgt cgatgtttca
      481 ccactgcgcc gcgtgaatca ggcaatttgg ctcctctgca ctggatcgcg tgaagctgcc
      541 ttccgcaaca tcaagaccat cgctgaatgt ctggcagacg aactgatcaa tgccgccaag
      601 ggctcgagca actcgtacgc aatcaagaag aaggacgaat tggagcgtgt ggccaagtcc
      661 aaccattaag gaagagacaa tattgttttg gaaaaatcca tcacgctgtt ggctttgttg
      721 aagtttctga tacatgctgt tatttt
//