LOCUS       CO961425                 557 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21h12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O02268 O02268 F45H10.4 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961425
VERSION     CO961425.1  GI:51334609
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 554.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtcctgatc tgtttgggtt ctgggtctcc agtggatggc gaaggagtgc tctggttcgt
       61 tacgctgtgt tcgctagtcg tcagtacgac cgcaacagtg ctcttcatgg tgaacaagga
      121 cggactcggt agagtcgctg ttgcaggagg attggtgcct tggaatgtta ttgagttcgt
      181 gtactcctcg attctcacac ttctctgcgg cgttgctttt tggcttagtt tcggatatgc
      241 tagccatgtt gagatcggcg gacactcagc tggatacgtg tttgccggaa tatttctgat
      301 cgcacaaatg gtttgctatg caatccccac cttgctcatc tacaaccgac tgcgcggtga
      361 gcaacacgag ctaatcgctg acatacaccc gttcggagtc agcaacgaca gcgccgccga
      421 acaacgcgac cagtcagcac cgtagtcagt cgatcgattc aaaacaaagt taaattcatt
      481 cttctcgaat acatatttta atcttcgatc acatttttct gagccaattt cattcatttc
      541 gggaatgtat gttaaaa
//