LOCUS       CO961408                 574 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21g05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q27511 Q27511 HISTONE H2A VARIANT. [1], mRNA
            sequence.
ACCESSION   CO961408
VERSION     CO961408.1  GI:51334592
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 577
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggcggcaag gcaagtcggg caaggacagc ggcaaggcga aatcgaagaa gatcatctcg
       61 cgcagtgcac gtgccggtct gcaatttcca gtcggtcgaa tccatcgatt cctgaagaaa
      121 cgcaccacca gcaacggccg agtgggcgca actgccgcgg tgtacagtgc ggctattctg
      181 gaatacctca ccgcagaagt gctggagttg gccggcaacg cgtccaaaga cctgaaagtg
      241 aagcgagtca ccccccggca cttgcacttg gccattcgcg gcgatgaaga actggactcg
      301 ctgatcaagg cgaccattgc gggcggaggc gtgattccgc acatccatcg ctacttgatg
      361 aacaagaagg gcgcccagca gcctggagca caaggaaagg gacagacagc ctgaggagcg
      421 aacggaaaat gaagttacga agcgacacaa caaacaatca ctactattta atcaatttga
      481 tgtctaacgt tccccgtact cacgccaagc attcacccca caattaatga gtaaacatgt
      541 aacaatttga aaaaaaaaaa aaaaaaaaaa caaa
//