LOCUS       CO961407                 758 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21g04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90860 P90860 F36A2.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961407
VERSION     CO961407.1  GI:51334591
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 782
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttgtacttc tcggcagagc tcgaacttcg aaatgatccc aatttctaaa ttggcttcac
       61 cactccaccc agcctcttcg ggtgccattc tccgtgtttt cgcgattcga aaatttgcca
      121 gtgctgtttt ggaacggccg ccaaaggcta agatccaact gaaaccacca ccaggcgaga
      181 agggctacat gaactatgaa cgtgactttt cacgcgaatc acgttataca aagccacata
      241 agaagaccga cacatttata agatttgtaa catttaacgc tcgccatcac atggagctct
      301 ggccgatcgt atttctctgc ggtgtctctt gttgcctagt cgtgggcata gcctacataa
      361 gttttgaaaa agtggaagtc tggcttgaca gaagcaccaa gaccccacca tgggattggg
      421 cgcgcattcg tgacaaatac tggagaatgc aaacagtatt tttcgatcca cttggcggga
      481 cccaccagcg tatagaaatc atggagaagc tacaggatga gatgttggag gcggccaagc
      541 agcgtggaac ccgcgatgcc ggtggctaca ttgttactaa ctagaacggg aaggccagca
      601 aagcagatgt aaagatgaaa cgaatgacca gagactaatt gaccattcat tgatcatttt
      661 ctggggcatc atttctggtc gtagtgtaaa aataaaaact gttctagtta aaaaaaaaat
      721 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaacaa
//