LOCUS       CO961404                 595 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O44953 O44953 C34B2.10 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961404
VERSION     CO961404.1  GI:51334588
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 596
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gataagtgat aacctgataa ctgaagaaaa aatcgcaatg gacagcctta ttaatttgat
       61 gccacagtgt attcgaaaca tcagttccca catcgattac gaaggacagc gaaatgcgga
      121 acgatttttc caagtgattt tgattgtctt gggcgttctg ggctttttgg tcggttactt
      181 cactcaacaa ctgtctgtca ccatgtattt tgttggcgtt ggttttctga tctcctgtct
      241 ggtagttctt ccaccttggc cattttatcg ccgcaatccg ctgaactggc aacaagatag
      301 gccaaaaaac tctgaggaag acaagccgga ttcttccact atgaatattg gaaagaactc
      361 aaaaaagagt cgttgaacta ttaattgatt taatgttcta tggcattttc aaggaaattt
      421 ctgttaaaaa attttagtta aaattattat ggaagaaagt acccaatggc tatatcgatg
      481 taacatttca atcttctcca tgggttatat attttgttcg aaaaagacga atactagtta
      541 tcgcataaaa tcccaaactt taaaaaaaaa aaaaaaaaaa aaaaaaaaaa acaaa
//