LOCUS       CO961403                 487 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21f11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element MER40 repetitive element, mRNA
            sequence.
ACCESSION   CO961403
VERSION     CO961403.1  GI:51334587
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 495
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttggctcttc ctcatccttt gtctcgcatt tgctgttgag tgcaggagat ctgaagacca
       61 aaaggagccc caccaccagg tgcgcgttca cttttccagg agggccaggt tcgcgccaat
      121 gatgaagctg ttcggctcta ggaagtcctc ccagacaaac aaggtcccca aaaaacactg
      181 ccgcaccaca gatcagtgtc ctggcggaac gcgctgcagg gcgggcattt gccagtgatg
      241 aagggaaacg aatgggcgtg ggactggggg agaggagaca ccaaacaatg gccgccaaaa
      301 gaacactatt ggaacacatt tgtttaggca ataccttgga gagagagaca ctttactcag
      361 cccacagctt ttgcttttcg ataaacaatt gggtgtccca aaaaaaaaaa aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaagaa gagaaaaaaa aaccccccaa aaaaaaaaaa aaaaaaaaaa
      481 aaaacca
//