LOCUS       CO961400                 572 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21f08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961400
VERSION     CO961400.1  GI:51334584
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 763
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agcggacttt ccgaaagccg attcaattgg gtgcattcct taacggtgtc agagctaatc
       61 cggctccaaa tggtccaaca cgtcaaatcg aaacgattgt cgttgaatgc aaatcgtcac
      121 gtcaaccacg aggaatggat tgtgtgatat tccggggtgg acatgcgaca cccgatcagg
      181 aacttgaagc ctttatcaaa actaaagtgg gacaacacaa tgtgcttatg cgtcaggaac
      241 gcgctgccgg taaaagaggc cgaccatctc tttccccgtt tcgtatgggc gacaaagcca
      301 atgtcaatga gaagggcgga gaaaaatcag gaggactttc ctaccatcta atagcggatt
      361 tggctgaaac acgcaatcca aatactccgg aagaacatat cgtgcgctac aacgctcatt
      421 ttgagtacat tgctccgcct gaatcatcca agcgccaggg gattaaagag catggaagtg
      481 acatgaaaga accaaaggga ggaggatttg cagtgggaga agctggtgga tctggataga
      541 gccaggtatg aagcgggacc tgatacattc ca
//