LOCUS       CO961397                 626 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21f04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961397
VERSION     CO961397.1  GI:51334581
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 720
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggtggatgag gctaagaagt tagcggacca gatgaagaag agtgcgtaac agatccagaa
       61 agaagtgcga gacaggatga actcctccga cccctacagt cgcgtcatcc accaactccg
      121 cacagctcac ttggtcgctt tgggcattga ggtgttcact ctctacctgg ggtggcgcga
      181 agtcaacttc atctggctgc tgttcccgtt gcttttctcg gctggagcga tttggattgt
      241 tgccgagcgt tggtatcacc aggcagatgg aagggcggac ttcaacaaat tgctgagtaa
      301 tgataagccg gctttgaggg gaagagcagg actagggctc ttgggtggat ttgtgctatc
      361 tctgcttgcc cagtggacct ctccgatatt tagctcatcg ctagggggac tgctgttcag
      421 tctctcaagt cacgccgctg ttctcatcgg cgcggtttgc tctgcgattg agctttacga
      481 aggcgtcaaa ctgaagaacc gttgaagtga ctaaaagcgc aaacctcgtt cttgtcaacg
      541 aatataagat ttaaaaatat cctttttcga ccgattgttt attttaaaat aggagcatag
      601 ccttccgcta aaaagcaaat gctcgt
//