LOCUS       CO961394                 677 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQF4_CAEEL Q09269 HYPOTHETICAL 16.0 KD PROTEIN
            C34C12.4 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO961394
VERSION     CO961394.1  GI:51334578
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 686
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgctacaca aaatggtcgg atccgtgcga atgcatcttc aaccatgaag cgattatgcg
       61 acggattctg tctatcttgc aagaagccca aaccgattgc accaacaccg aatgttcgac
      121 cgggccttcc gatgctggtg ctggcggttc gaacatgatg ctcattgcca tggcttgggg
      181 actgttcgcg atgttcatgt tcatgatgcg tccggcctcg atgcgcagct cgaccggggg
      241 aaacgagggt gacgctgcac aggagaaggc gccttccaat tcgtccagca ataacaacga
      301 caacaacgga ccgcctcccg gtgatgtgtt ctgagttggc cgcggagaaa cacggtgctt
      361 gcgcgaagtg cccggacccg gatgaaaaaa aaccaaacac atcttcgtcc cttcatctgt
      421 ccccaccact tctcaaattg ggtaacatgt acagcaacca aaaactatcc cggatattca
      481 aaatcacccc tagttagatg gatttagtta cgcactttcc gtccacccca aatccaagaa
      541 attgattcta tttatttttt gttctcctgt ccagtctaca catttcctga atactaaaga
      601 attgtattaa ctatgtaaat gcacttttgt taaaagataa atggtttttc tttcaaaaaa
      661 acaaaaactc gagacta
//