LOCUS       CO961386                 782 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21d10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q22782 Q22782 SIMILAR TO RAS-RELATED PROTEIN. NCBI
            GI: 1125833. [1], mRNA sequence.
ACCESSION   CO961386
VERSION     CO961386.1  GI:51334570
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 791
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttaaatcca tgtcttctga ttttggaaat cccttgaaga aatttaaatt ggtacttctt
       61 ggagagcaaa gcgttggcaa gacttcactc attacaaggt tcatgtacga cagttttgac
      121 aatacatatc aggcgaccat tgggattgat tttctcagca agacgatgta tcttgaggac
      181 agaacggtta gacttcagct ttgggacacc gcaggacagg aacggttccg ttcactcatt
      241 ccatcctata ttcgcgattc taccgttgcc gtggtcgttt acgacatcac aaatgcaaat
      301 tctttccacc agacttcgaa atggattgat gatgtgcgca ccgaacgggg cagcgatgta
      361 attataatgc tcgttggcaa taaaacagat ctggctgaca agagacaagt ttctacagaa
      421 gagggtgagc agaaggcaaa ggagctgaat gtcatgttca ttgagacatc ggccaaagca
      481 ggctacaacg tgaaacagtt gttccgaagg attgccagtg ctcttcctgg aattgaagtg
      541 gagaagtcat ctgatgcgaa gcaagttgtg gacattgggc caatcaagcc acgccagatt
      601 gtgaccgaag aaggatcgtg ctggtgttaa gcgccgggat gcggcaaaac acaatccttt
      661 taaatgtgga cctttttgaa attgattttt acgattaatt ttgattatta atttaattta
      721 tggacctatg tatttgttgt aaaagaaaca aactcgttta tgaaaaaaaa aaaaaaaaaa
      781 aa
//