LOCUS       CO961380                 557 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21d01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YWY1_CAEEL Q11088 HYPOTHETICAL 19.2 KD PROTEIN
            C01C4.1 IN CHROMOSOME X PRECURSOR. [1], mRNA sequence.
ACCESSION   CO961380
VERSION     CO961380.1  GI:51334564
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 567
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atcgcttctg tcctgctgat tttctctgtc gccgaacaac ttgatacgac caattccgca
       61 gcaccaccac acacccctcg ccccctgatg agcatggacc gaaatgcatt tcgcatgagt
      121 tttggaaaga agaggacagc tcagagtccc atgtcgcctt tggacagaaa tgtgttccga
      181 atgagcttgg gaaaacgggc gcccgcaata aagccgagtt ggtacgatgc aataaagaaa
      241 gagcaggagg acgacgctcg tcagagaaat ggccatcaac aaaacaacaa cagtccctcg
      301 ctaaaaatga aaatggaccg aatcatggaa ccgcaatggc gacaaaagct gttcaacaag
      361 agtctggacc gatggtactt cggaggaaag cgatgatggg gaacaatggc aaagtgaccg
      421 gcgaccattc caaattccaa acacatgtag ataaatgtga gcccgcattt gccgtaatgg
      481 cagaggcaga cataatagca gcatacaatt gaccggtcaa taaatattag tggattaaaa
      541 aaaaaaaaaa aaacaaa
//