LOCUS       CO961378                 515 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21c09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO961378
VERSION     CO961378.1  GI:51334562
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 642
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcctggatca agaaaatgag cggcgagaat atcagcacca acggcgaccg tgagaacttc
       61 tacaaattgc taaaagacgg cacactactg tgcaagctgg ccaacgggat tcaggcgggc
      121 tccgtcaaga agatccagca gccgactggc aacttcgcct gcatggagaa catcaacgct
      181 ttcgtgtcgt tcgcgaagag ccagggagtg cccaccgagg agaccttcca gagcgtggac
      241 ttgttcgagg cacgtgacct ctactccgtg tgcatgaccc tgttgtcgtt gggccgaatt
      301 atggagaaga agggaaagcc gaacccattc tctggatgaa gagtagaagt gagtgcagcc
      361 cacgacggac agagcatgtg ctatcgctcc cattcgagaa ctccccgttt tgcgaatttt
      421 ctccccgtgt gcgaccttgc aacaataccg aggcgttaac tgttttcccc tctctctcct
      481 ctcaaactgt gggcatttga aaatagcgat gccgg
//