LOCUS       CO961362                 594 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21b02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:MTHR_CAEEL Q17693 PROBABLE
            METHYLENETETRAHYDROFOLATE REDUCTASE, mRNA sequence.
ACCESSION   CO961362
VERSION     CO961362.1  GI:51334546
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgttatttgc agcgaaacgc gagcatttta cagcagagca tctaacaaag cagaacaaga
       61 gaagtttagg cataaaatgg ttttggcgcg cgagatcgcg aaaagagtac gcgacgacgc
      121 agtgttcttc tctttggagt tctttccgcc ctactccagc cgactggatg acatcgaacg
      181 gttcgaggag acactggact ggctcaacag ctcctctccg ctgtttgtca gcatcacttg
      241 gttcttgcgc aacaatcccg gaaatctcgc cataaccaac tcatcttccg ccatcgctgc
      301 gctgactaag aacagacacg ggattgagac gttgctgcat ttgacgcggt cgaacggggc
      361 atccacaatg tgttggcttt gcgcggcgac gaccacaaca gcggggagac gcagaacaac
      421 gacttctacg cactgcactt ggttaggtgg gcacggcagc tggagcagag cagggcaaac
      481 gggccgttga cggtggccgt ggcggggcat cccaccggcc atccggccgc caacagctac
      541 gaggagacgc tggaccacct ccgccaccag gtggacgccg gcgccgactt cgtg
//