LOCUS       CO961361                 552 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe21b01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O02080 O02080 SIMILAR TO CCAAT/ENHANCER-BINDING
            PROTEIN. [1], mRNA sequence.
ACCESSION   CO961361
VERSION     CO961361.1  GI:51334545
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 570
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cacgaaaacg ccaaaatgtc cgcccaacgg agcggccgaa agcggaacgc ggcacagcaa
       61 tcggcggacg aggcaactgg acaaccgcaa cagcagcaca actcacccgg acagcaatgg
      121 agtccggcga gcagcaacca ggagcagctg gacgagtacg cgttgaagag gttgcgcaac
      181 aacgaggcgg tgagcagaac gcgccagaaa aagcgcctgg agcaacagca cacctcgcaa
      241 cgcgtgaagg agctgcgaga ggagaatgcc cagctggaga ggacactgga cacaatgcga
      301 cgcgagttgg ccctgctgaa ggagatggtg gtggtgtgtg cggccaaggg cgacgagggg
      361 agcagcaaca ggtgatggat ggtggcggtg gacgaacgaa ttctattagt acaagaggca
      421 catacataca tatcttatgt gattgaaccg aatgatggat acggaaacga tcagccaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaactca aaaaaagttc
      541 tctcaaacgg ga
//