LOCUS       CO961352                 517 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20h10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18231 Q18231 SIMILAR TO 40S RIBOSOMAL PROTEIN
            S30/UBIQUITIN-LIKE PROTEIN. [1], mRNA sequence.
ACCESSION   CO961352
VERSION     CO961352.1  GI:51334536
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 518
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggaagtaga aggttccgac actatcgaga atgttaaagc caaaatccaa gacaaagaag
       61 gcattccacc tgaccaacaa cgattgattt tcgccggtaa gcaactcgag gatggacgaa
      121 ctctagccga ttacaacatc caaaaggaat cgaccttaca cttggttttg cgccttcgcg
      181 gaggaaaggt ccatggttcg cttgctcgtg ccggaaaggt tcgcgctcag acaccaaagg
      241 tcgaaaagca agaaaagaag aagaaaaagc gcggacgtgc attccgtcgt attcaataca
      301 accgtcgcta cgtcaatgtt gcaatggctg gaccaggccg caaacgcgga cccaactcga
      361 atgctgcgta acttggaaaa gattaaatgg attcattcgc atgaattgat gtttttgcag
      421 gaatcctttt tattttctgt tatttgaaat tcgataaacc aatttgttgc aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaccaaa
//