LOCUS       CO961351                 706 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20h09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:OV21_ONCVO P29778 SMALL HEAT SHOCK PROTEIN OV25-1,
            mRNA sequence.
ACCESSION   CO961351
VERSION     CO961351.1  GI:51334535
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 807
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aaagttctat cgcgacccaa tggctctgtg gttctacgac ccgtttcgcc cgctgatgcg
       61 ccgtcgcgcc tttgtggacc cgttcgcggc gctggaccac ttcttcgacg acaatctggt
      121 gccgcagatt cgcgaaaatg cggccaagct ggacattgac gataaggggc tgctcacgta
      181 cacggtggac acggccggct atcgccccga ggagctgcaa gtgaacgtcg aaggcgacga
      241 gatcgtcgtg cagggcgagc acaaacagca aaacgagaat gagaccgtgc atcgccagtt
      301 cattcgccgt gtgcgcattc cgaacggcta ccagaaggag tcgctcaagt gcgagatgga
      361 cgccagcggg cgcctgagtc tgtgcgcaca gaaggcccag aacgggaagg aggagtcgcg
      421 cagcattccc atcgagatga agaaggcggt cgaggagaag tgaaggagtg aagaacggca
      481 aatggggatg aaggcaaaat ggattggata attgcccgac ttctaagttc ttgtctttta
      541 aagtctaaac aatcaacggc ttcattgatt catgaatcat ggctctcttt aaagtttaaa
      601 ttaaacatac atctcccttc tgtcatctct atgtctatgg catccatcca tcatccattt
      661 cattgctatc aactatcaca aatcaattca tggaattcat gatgga
//