LOCUS       CO961348                 631 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20h05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains PTR5.t3 TAR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961348
VERSION     CO961348.1  GI:51334532
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 887
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgtcgttgt tctggccctc gccctgttgg cggccgtcgc ccacagtgcg ccgattgctg
       61 aggagcccca gcagcaggac cagtcctccg cctccgacga cgggaccctg agcgcgcagg
      121 agatcaccgc gaagctggac gagttggcgg accaggtgca ctccaccctg ctccagtacg
      181 tggaccgcta cgtggacgcg gacgactcgc tggccgccga actgctcgac cagtgcacca
      241 agcagtgcga ccagctgccc cagctggcac agcgcgccct ccagtccttc aaacagatgc
      301 cggacgagca caagcagacg ctgaaggcga cgctgcgccg ctctttcgac gagctgatgc
      361 agttcggcgt caaattcatg gacgtgtggg cgcccaagtt cgacaacgcg ctgaccgaca
      421 aggtggtctc cgttctggtc aagcaggcgg cagacaccac tcgcaaggac ctgttgaacc
      481 gcatcgacca gttggacgtg gacgccgcag cgaacgctct caacgacctg ttggcggaca
      541 aggtgcccca gatgtgccac acccactgcg acatggccca caagcgcgtc cgggacgact
      601 tccagcgcct ccaatcgacc tcggacgagg g
//