LOCUS       CO961345                 557 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20h01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RPB8_CAEEL Q19826 PROBABLE DNA-DIRECTED RNA
            POLYMERASES I, II, AND III 17.1 KD POLYPEPTIDE, mRNA sequence.
ACCESSION   CO961345
VERSION     CO961345.1  GI:51334529
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 580
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgaacagcat ctatttctaa gacctgttta cggtgaagaa catagacccg gatggcaaga
       61 agttcgaccg agtcagccga ctcttctgcg acagcaagtc cttcaagatg gatcccattc
      121 tggacgtgaa cacgcaactc taccaaatgt gcatcaacga caagttccgt ctgatgctct
      181 gcacatcggt ccgggaggac gaactcgcgg acgagggcga gtacgacaaa tcgatcgaga
      241 acgtccctcg ggtgtggatg ttcgaatacg tgatgtacgg gaagatctac aagatcgagg
      301 ccgacgacag cagcaatccc gagaccaatc ggatggcggc ctacgtctca tttggcggac
      361 tgttgatgcg aatccagggc gaggccaaca acctgaacgg attcgaagcg gacaggaata
      421 tttacttgct actgaagaaa gttccaaact aattatctca acactcgttc gtttgtttgt
      481 tctcatcaga atattaaatc tgggaattat ttacttttat gtatcgttaa aatttaaaaa
      541 aaaaaaaaaa aaacaaa
//