LOCUS       CO961339                 793 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20g06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O45764 O45764 T06E6.10 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961339
VERSION     CO961339.1  GI:51334523
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 815
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgatcctcg caacttttcc caccccgcaa ttcccccatt tcctccattc atcattcggc
       61 tcgcttctcg acacctttca ccatccgccc acgaacgctt tgtttacaca tccaatggct
      121 gcaatggatt gttttccaaa tattatagaa aacttttggc gccagattgg gatgacgaag
      181 ggacagcggc ggcggacagc agcaggtgag ctgaggtgaa agaagcgtgg gcagaggacg
      241 gatgggggct aaggacacca ctttttgggc aaatatccaa tccatatcca gagaggaagt
      301 ggtggtgctg agaacacgag agcgggggca acgcgaaaga gagaggaagg agccagagcc
      361 agagcaatcc cccatccatt tccattccat tccacacaaa tttttccatt ggcgccaaca
      421 cgctggtgaa atgaaggagg gcgagaagag cgcagcgccc aagggaggga taatgcacga
      481 caactcgcaa ttcaattggc tgacttcccg cgctcagaat gcgcccgtat tcgctgtgcc
      541 gccggcgagg agtgccgact ggtgcgcgtc cagtgcatcc gtgccccctg tcccccgttg
      601 cccatgtgcc gtcccatcgc gaatccgttt ccgcccgaac aaagaagagt ggtggccacc
      661 gtccgctgga agcgccatcc ccaatgaatc catttgtgtt cggtgacgtt gtttccgctg
      721 acaacggtga cgttgtttcc gctgacaacc cgtttccgta atcttacaca acaaatacta
      781 cttaacatgc aaa
//