LOCUS       CO961336                 629 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961336
VERSION     CO961336.1  GI:51334520
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgctgcaag gtcccctgaa agcagaaaag gcatcactga atgtcaacaa cgagccattt
       61 tatttgaatt tcgacaattt tccggcggta tgaaagcgca aaatggaaaa aaaaaaaaaa
      121 aaagaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaccaaaac ccgaaaaatt tttcccccca
      241 gggggaaatt tcggaaaatt ccctcaaaag ggggggcccc ccaacctggc tttaaagggc
      301 cccattcccc ccaaaggggg ttctattaaa attcccgggc ccgcttttta aaacctccgg
      361 gcggggaaaa ccccggggtt tccccatttt atccccttgg ccccaatccc ccttttcccc
      421 gcgggggtaa aaacaaaaaa gccccccccc tttccccttc cccaaatttg ccccccccga
      481 aggggaaaag gacccccccc ggaaccgccc cttaacccgg gggggggggg ggggttcccc
      541 ccacggggcc cccaacattt gccgccccct aacccccccc ccttttcctt tttttccttt
      601 cttttccccc aagtttcccg ggttttccc
//