LOCUS       CO961333                 556 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20f10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL2A_RAT P18445 60S RIBOSOMAL PROTEIN L27A, mRNA
            sequence.
ACCESSION   CO961333
VERSION     CO961333.1  GI:51334517
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 557
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcgatggcca ccaccaaaaa gaagacacga aagctccgcg gacacgtctc gcatggacac
       61 gggcgcatcg ggaagcaccg caagcacccg ggcggtcgcg gaaacgccgg tggccagcac
      121 caccaccgga tcaacttcga caagtaccac ccagggtact tcgggaaggt cgggatgcgc
      181 aacttccacg tgcgcaagaa ccactactac tgtccgacga tcaacctgga caaggtgtgg
      241 tccctggtca cggagcggac gcgcgccatg tacgcggacg tgaaggacaa tgcgccggtg
      301 attgacgtag tgcgggcggg ctaccacaag gtgctgggca agggactgct gcccaagcag
      361 ccggtgatcg tcaaggccaa gtacttctcg cactcggccg aagagaagat caagaaggcg
      421 ggcggcgcat gtgtgctagt cgcctgagcg ccagcagaaa gtgatgatga acattgttgt
      481 tgttttgata aacaaattga gatgaaacct tgattcaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa accaaa
//