LOCUS       CO961331                 648 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20f07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YXX3_CAEEL O01578 HYPOTHETICAL 16.3 KD PROTEIN
            F53F10.3 IN CHROMOSOME I. [1], mRNA sequence.
ACCESSION   CO961331
VERSION     CO961331.1  GI:51334515
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 657
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttttgtgata gttatttatt ttccatcaaa cagaaatggc ttctgcacta tacaatacac
       61 tttgcaaaat tggagacaag gctgtctatc ctttgcttcc tgcttttgcg aagccagcat
      121 ggaatcatcc ggccggtccg aagactgtgt tcttctggtc gcccacaatc aaatgggtgc
      181 ttgttggtgc gggacttagt gatcttgcca gaccagctaa caaactgagc ttgaatcaga
      241 atgcagcact actcgcaact ggctcaattt ggacgcgcta ttgttttgtg attgtgccga
      301 agaattatta cttggccagc gtaaacttct ttgtcggccg cacaggactt gttcaatgca
      361 tccgggttct tcactatcag tacacccaca aaaatgaagc cgaagctatt gtgaattaga
      421 gaagacagaa gacacagtgc cttcctgtga tccaattctc catttcttag ttttgtagca
      481 ctttttattt ccacatttta atcctcatcc gaatacgggg cttgttcgat tgagaaacga
      541 caaatattct aggtcacaca tttttgcaaa aaaaattagc tcagatatga cgaataaaga
      601 tttattacca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaccaaa
//