LOCUS       CO961328                1447 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20f02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains MER22.t1 MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961328
VERSION     CO961328.1  GI:51334512
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 704.
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccagaacacg ttgagccggc agagagacca tctggtcggc cagccctgaa aaggaggatg
       61 taaatgaaag gtctacaaag tcgaataaat tgagtgcatc aaataaagat gttacatcta
      121 atgcatcttt agtcaatggt aaaacttgaa aatgcaaaaa aaaaaaaaaa aaaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      241 aaaaaaaaaa aaaaaaaaca aaaaccccaa aactttttcc ccccaagggg aaatttcgca
      301 aaaatccctc aaaagggggg cccccccaac aaggatttaa agggccccat ttcccctaaa
      361 aggaattcaa ataaaatttc cgggcccttt tttttaaact ttcggaaggg gaaaaccctg
      421 ggttttcccc actttatccc cttggcccca atcccccttt tccccccggg ggtaaaaaca
      481 aaaaagcccc cccccattcc ccttcccaaa aatttccccc cccaaaaggg aaaaggaccc
      541 cccctttaac cgccccttaa accccggggg ggggggggtt tccccccccc gggcccccaa
      601 cctttccccc cccccaaccc cccccccttt tctttttttc cctttctttt cccccccgtt
      661 tcccgggttt tccctttaag ctttaaaatc gggggcccct tttgggttcc caatttatgg
      721 ctttacggcc cctcacccaa aaaaaatttt ataaggggat ggtttcccgt ttgggccccc
      781 cccccaaaaa aaagtttttt tcccctttta aatgaaaacc cattttttta aaaggaaccc
      841 ttcttccaaa cggaaaaacc ctcaccctat tctggttttt ttttttattt taagggaatt
      901 cccaattttg cctttggtaa aaaaacaaat ttaaaaaata ttaccaattt taaaaaatcg
      961 ggccaagggc actaaaaaac aaaaaaataa aacactccaa aacgttaacc caaaaataat
     1021 cccagacaat aaaaaagaaa accccaaaaa aaagtgattc attgaatctg ataaacccag
     1081 cgttattcac caacaataac cgggccctat tatgacacaa aagaatttaa aaaatagaca
     1141 tataaaaatt cttaatataa agattggtcg aatataaaac ctacaaannn naaagctgaa
     1201 atagtactaa caatacacta aataactaat aaatgatata tcattctatt ataaactata
     1261 ataaaagaat caataattaa aataaaaata aaatataata ttatacttta cannnannaa
     1321 tcaccattaa acataacgca cctctagcaa gataatatta aattaacaaa cccaaccaca
     1381 aaaacaaacg aaaacaatca ccaaataaaa gctctctaac acntaacgga agtcgtacat
     1441 agcgaaa
//