LOCUS       CO961325                 539 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20e10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:H2B_SIPNU P30757 HISTONE H2B. [1], mRNA sequence.
ACCESSION   CO961325
VERSION     CO961325.1  GI:51334509
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 574
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gaaacactcg aaccaaaaat ggcgccacac aagccgtccg gcaagggagc gaaaaaagcc
       61 gtgaagtcga ccaaggcggt gcgcaccggc gacaagaagc gccgaaagtc gcgcaaggag
      121 tcctactcgg tgtacatcta ccgcgtgctg aagcaagtgc acccggacac tgggatctcc
      181 tccaaggcga tgtcgatcat gaactcgttc gtcaatgacg tgttcgagcg catcgctgcg
      241 gaggcgtcgc gtctggccca ctacaacaag cgctcgacca tctcgtcccg cgaaatccag
      301 accgcggtgc gcctgatcct gccgggtgag ctggccaaac acgctgtctc ggagggaacc
      361 naggcggtta ccaagtacac ctcgtccaag tgaggggtcg gcaggtgaaa gtgggccaca
      421 gcagcagaaa gggacaacgt tttcatttta aattatgtaa aaaagagagc aattttgact
      481 acagttggtt atataaaatg taaaataaac tgattcagtc gaaaaaaaaa aaaaacaaa
//