LOCUS       CO961323                 633 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe20e08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961323
VERSION     CO961323.1  GI:51334507
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 638
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tacggcattt atcgctcggc atcggtgagt cgggtgccgg aaaagagcga tgccccggtt
       61 aaggcggcgg aaagaggcgt ctcgttgccc cgctccccct ccgtgtcgca catccccgca
      121 ttcggcgttg ccggcgccac ctacatgccg ttcaccggct tctccgcagt caggaccagc
      181 gtctatcgca ccaacccatt cttctcgctg agaagctaca tgcaggacaa gtactactac
      241 ttctcgcacc gctacaagcg cacccaattc caccgacact actaccacgg tcaagtcgag
      301 aactacccgt acagatggaa cttcgtcccc aagggcaccg ccaccggcca gttctattat
      361 gccagacgag tccaattcga tcccgtccag tatgggctgc tgcgctatta tgacccctac
      421 cgtcgctact acgatccgta ccgcgactcg tacatcgaat tgtacaacta cagacccaaa
      481 ctgatggaca gcctctattg aagtgcccag aaccgaatgt tagaaagatg gatggaatgg
      541 atttgaacgc gataaacaga agccattgtg gatgggaact cctctgtttg tttgttcagc
      601 gaaccctcga ttgagcaaaa aaaaaaaaaa aaa
//